Development and application of non-targeted LC-MS/MS for human biomonitoring
Individuals are exposed to a complex mixture of chemicals in their daily lives through the environment, products, food and drinking water and at work. Human biomonitoring allows us to measure our exposure to chemicals by measuring either the substances themselves, their metabolites or markers of subsequent health effects in body fluids or tissues.
With the objective of addressing knowledge gaps and promoting innovative approaches, The European Human Biomonitoring Initiative, HBM4EU, has been launched. HBM4EU is a joint effort of 26 countries, the European Environment Agency and the European Commission, co-funded under Horizon 2020.
The main objective of the initiative is to coordinate and advance human biomonitoring in Europe. HBM4EU will provide better evidence of the actual exposure of citizens to chemicals and the possible health effects to support policy making.
We are involved in work package 16 dedicated to the development of analytical workflows enabling the detection of emerging chemicals with non-targeted LC-MS/MS.
Different aspects of the analytical process were discussed in the following publications:
Caballero-Casero, N., Belova, L. Vervliet, P., Antignac, J.-P., Castaño, A., Debrauwer, L., Esteban López, M., Huber, C., Klanova, J., Krauss, M., Lommen, A., Mol, H. G. J., Oberacher, H., Pardo, P., Price, E. J., Reinstadler, V., Vitale, C., van Nuijs, A. L. N., Covaci, A. Towards Harmonized Criteria in Quality Assurance and Quality Control of Non-targeted LC-HRMS Analytical Workflows for Emerging Contaminants Identification in Human Biomonitoring Trends in Analytical Chemistry, 13 (2021), 116201.
Oberacher, H., Sasse, M., Antignac, J.-P., Guitton, Y., Debrauwer, L., Jamin, E. L., Schulze, T., Krauss, M., Covaci, A., Caballero-Casero, N., Rousseau, K., Damont, A., Fenaille, F., Lamoree, M., Schymanski, E. L. A European Proposal for Quality Control and Quality Assurance of Tandem Mass Spectral Libraries Environmental Science Europe, 32 (2020), 43.
Pourchet, M., Debrauwer, L., Klanova, J., Price, E. J., Covaci, A., Caballero Casero, N., Oberacher, H., Lamoree, M., Damont, A., Fenaille, F., Vlaanderen, J., Krauss, M., Sarigiannis, D., Barouki, R., Le Bizec, B., Antignac, J.-P. Suspect and Non-targeted Screening of Chemicals of Emerging Concern for Human Biomonitoring, Environmental Health Studies and Support to Risk Assessment: from Promises to Challenges and Harmonisation Issues Environment International, 139 (2020), 105545.
Oberacher, H., Reinstadler, V., Kreidl, M., Stravs, M. A., Hollender, J., Schymanski, E. L. Annotating Non-targeted LC-HRMS/MS Data with Two Complementary Tandem Mass Spectral Libraries Metabolites, 9 (2019), 3.